Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.是什么意思？

Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.

方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

methods

n. method的复数：方法，方法( method的名词复数 )，方法论，教学法，分类法；

例句
The old methods weren't adequate to meet current needs...
老一套方法已不足以满足当前的需要。

the

art. 指已提到的人（物），指说话人与听者已知的人（物），用于独一无二的事物前，与形容词最高级和序数词连用；

例句
He did have a real knowledge of the country...
他对该国的确知之甚多。

restriction

n. 限制，限定，拘束，束缚，管制；

例句
The second restriction upon the president's power is the limited time at his disposal.
总统权力受到的第二项限制就是可以自由支配的时间有限。

endonuclease

n. 核酸内切酶；

例句
Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

and

conj. 和，与，而且，于是，然后，因此；

例句
I spend a lot on expensive jewelry and clothing...
我花很多钱购买昂贵的首饰和衣服。

DNA

abbr. did not attend 没有参加，does not answer 不回答；

例句
Genetic abnormalities are more often associated with faults in paternal DNA than in maternal DNA.
相对于母本DNA,基因畸变往往更多地与父本DNA上的缺陷相关。

ligase

n. 连接酶；

例句
Methods: The restriction endonuclease and T 4 DNA ligase were used to construct the vector plasmid.
方法载体的构建采用限制性内切酶酶切、4DNA连接酶连接等方法.

were

be的过去时复数和第二人称单数形式，有时代替 was,用于条件从句、动词 wish 之后等；

例句
Shops, houses, and vehicles were set ablaze.
店铺、房子和车辆全都陷于火海。

used

adj. 用过的，习惯于，二手的，旧的；v. 使用（ use的过去式和过去分词），常常，经常；

例句
The gym can be used by both able-bodied and disabled people.
这家健身房健全和残障人士都可使用。

to

prep. 向，朝着，到，关于，属于；adv. 朝一个方向的，到某种状态，关闭；

例句
I suggest you leave it to an expert...
我建议你把它留给专家来解决。

construct

vt. 修建，建造，构成，创立，[数]作图；n. 结构（物），构想，概念；

例句
You will find it difficult to construct a spending plan without first recording your spending...
你会发现不先把自己的开销记录下来就很难制订支出计划。

vector

n. 矢量，航向，[生]带菌者，[天]矢径；vt. 用无线电引导，为…导航；

例句
In this paper the idea of synchronous vector and adjoint matrix are proposed.
本文提出了同步矢量和伴生矩阵的概念.